PAP pen from Abcam

Product Specs
ItemPAP pen
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab2601
Quantity1 U
TargetPap Pen

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Fine-needle percutaneous muscle microbiopsy technique as a feasible tool to address histological analysis in young children with cerebral palsy and age-matched typically developing childrenPLOS ONENovember 22, 2023Jorieke Deschrevel, Karen Maes, Anke Andries, Nathalie De Beukelaer, Marlies Corvelyn, Domiziana Costamagna, Anja Van Campenhout, Eva De Wachter, Kaat Desloovere, Anouk Agten, et al.min at room temperature in a humid chamber and the muscle sections were delineated with a PAP pen (ab2601, Abcam). The samples were then blocked for one hour at room temperature with 10% normal goat serum in

(2) A differentiation protocol for the generation of pancreatic beta-like cells from human embryonic stem cellsSTAR ProtocolsSeptember 1, 2023Xisheng Li, Zhangjing Ma, Kathy O. Luimedium,Polysciences,Cat# 18606 Superfrost plus slides,Fisher Scientific,Cat# 22-037-246 PAP pen,Abcam,Cat# Ab2601 Leica DM4 M fluorescence microscope,Leica,https://www.leica-microsystems.com/products/light-microscopes/p/leica-dm6-m/ BD

(3) Immunoexpression of neuropilin-1 in the chorionic villi of HIV-infected preeclamptic South African women of African ancestryHistochemistry and Cell BiologyJune 11, 2023Nitalia Naidoo, Tashlen Abel, Jagidesa Moodley, Thajasvarie Naickersolution (5 min). Following this, the sections were circled with a delimiting hydrophobic pap pen (ab2601, Abcam, UK). To quench endogenous peroxidase, tissue was placed in hydrogen peroxidase-blocking reagent

(4) Evaluating mitochondrial length, volume, and cristae ultrastructure in rare mouse adult stem cell populationsSTAR protocolsMarch 17, 2023Triolo M, Wade S, Baker N, Khacho M.Software,https://imaris.oxinst.com ,, Other,Other,Other ,, Immu-Mount Mounting Media,Epredia,Cat# 9990402 Pap Pen,Abcam,Cat# ab2601 Charged Glass Slides,Fisher Scientific,Cat# 12-550-15 Cover Slip (24 × 50 mm),Fisher Scientific,Cat#

(5) Membrane dual-targeting probes: A promising strategy for fluorescence-guided prostate cancer surgery and lymph node metastases detectionActa Pharmaceutica Sinica BMarch 1, 2023Ling-Ling Wu, Qinxin Zhao, Qinghua Wang, Qingyang Zhang, Feiya Yang, Bo Zheng, Hai-Yu Hu, Nianzeng Xingtimes with PBS. The cryosections were dried, and the tissue to be stained was circled by the PAP pen (ab2601, Abcam). Then, the samples were blocked with 200 μL anti-goat serum at room temperature for 60 min.

(6) Early evolution of glial morphology and inflammatory cytokines following hypoxic-ischemic injury in the newborn piglet brainScientific ReportsJanuary 6, 2023Elliot J. Teo, Kirat. K. Chand, Stephanie M. Miller, Julie A. Wixey, Paul B. Colditz, S. Tracey. Bjorkman20 min, Biocare Medical). A hydrophobic barrier was drawn around the tissue using a PAP pen (Abcam; ab2601) followed by non-specific blocking with 5% donkey serum (Sigma–Aldrich; D9663) in PBS with 0.5% Triton-X 100

(7) Mitochondrial dysfunction compromises ciliary homeostasis in astrocytesJournal of Cell BiologyJanuary 2, 2023Olesia Ignatenko, Satu Malinen, Sofiia Rybas, Helena Vihinen, Joni Nikkanen, Aleksander Kononov, Eija S. Jokitalo, Gulayse Ince-Dunn, Anu Suomalainen2 × 5 min 100% EtOH), and dried at room temperature (5–10 min). A barrier was drawn with a PAP pen (ab2601; Abcam). Tissue sections were treated with 20–30 μl per section of Protease IV for 20 min at RT, rinsed

(8) Progressive pulmonary fibrosis in a murine model of Hermansky-Pudlak syndromeRespiratory ResearchDecember 1, 2022Shachar Abudi-Sinreich et al.s, to 100% ethanol for 3 min and then air dried. Hydrophobic barrier was drawn (PAP pen, Abcam, Cat. ab2601), then 5 drops of Protease Plus (ACD, Cat. 322331) were added to digest the lung tissue at 40º for 30

(9) An optimized protocol for PD-L1 pathological assessment with patient sample deglycosylation to improve correlation with therapeutic responseSTAR ProtocolsMarch 18, 2022Ying-Nai Wang, Heng-Huan Lee, Yongkun Wei, Yu-Yi Chu, Weiya Xia, Michael Wang, Dihua Yu, Mien-Chie HungMicroscopy Sciences; Cat#71310), similar pens are available from other manufacturers, such as Abcam (Cat#ab2601) or Thermo Fisher Scientific (Cat#008899).e.Add enough of the appropriate solution to cover specimens

(10) Diverging prefrontal cortex fiber connection routes to the subthalamic nucleus and the mesencephalic ventral tegmentum investigated with long range (normative) and short range (ex-vivo high resolution) 7T DTIBrain Structure and FunctionJanuary 1, 2022Volker A. Coenen et al.X-100) for 2 h. The edges around the slides were dried, and using a hydrophobic pen (PAP pen, Abcam-ab2601) a barrier was drawn around the section to limit the required antibodies used. Sections were incubated

(11) Neurogenic-dependent changes in hippocampal circuitry underlie the procognitive effect of exercise in aging miceiScienceDecember 17, 2021Xiaoqing Alice Zhou et al.2011",(http://www.imaging.org.au/AMBMC/AMBMC) SPSS,Microsoft,26 ,, Other,Other,Other ,, 9.4 T horizontal bore animal scanner,Bruker BioSpin GmbH,N/A PAP pen,Abcam,Ab2601 APA apparatus,Biosignal,N/A MR-compatible rectal temperature probe,SA Instruments,SAII infrared pulse

(12) "An exercise ""sweet spot"" reverses cognitive deficits of aging by growth-hormone-induced neurogenesis"iScienceNovember 19, 2021Blackmore DG, Steyn FJ, Carlisle A, O'Keeffe I, Vien KY, Zhou X, Leiter O, Jhaveri D, Vukovic J, Waters MJ, et al.Excel,Microsoft,16.53 Prism,GraphPad,9.0 ,, Other,Other,Other ,, Running disc,Med Associates,ENV-047 PAP pen,Abcam,Ab2601 APA apparatus,Biosignal,N/A Osmotic mini pumps,ALZET,1004 Infusion cannula,Plastics One,3280PM-SPC

(13) GAK and PRKCD are positive regulators of PRKN-independent mitophagyNature communicationsOctober 20, 2021Munson MJ, Mathai BJ, Ng MYW, Trachsel-Moncho L, de la Ballina LR, Schultz SW, Aman Y, Lystad AH, Singh S, Singh S, et al.room temperature for 3 min each. The area of interest was circled by a hydrophobic PAP pen (Abcam, ab2601), and the slides were placed in a humidified chamber to avoid drying out. The sections were subsequently

(14) ImmunoCluster provides a computational framework for the nonspecialist to profile high-dimensional cytometry dataeLifeApril 30, 2021James W Opzoomer et al.optimization for your tissue type Hard slide box for storage in −80°C (VWR #631-1504),"PAP pen (Abcam, #ab2601)","FcR block (Trustain, Biolegend, # 422302)",100%,5% Pipettes,,"Xylene (Acros, #180862500)",, "Plastic

(15) Localization and expression of ADAM2 in the dromedary camel testis, epididymis and sperm during rutting seasonAnimal ReproductionApril 7, 2021Abdulkarem Al-Shabebi, Thnaian Althnaian, Khalid Alkhodairfluorescent microscopy. Sperm-smeared slides were surrounded by the PAP pen (Abcam, Inc., cat. no. ab2601). Slides were incubated in PBS for 5 min, 70 μL of normal goat serum (NGS) (Abcam, Inc., cat. no. ab7481)

(16) Acetylcholine production by group 2 innate lymphoid cells promotes mucosal immunity to helminthsScience ImmunologyMarch 4, 2021Luke B. Roberts et al.were 537 washed with distilled water. Next, tissues sections were marked using a hydrophobic pen (PAP pen, 538 ab2601, Abcam) to prevent leakage. Tissue sections were incubated with blocking buffer (1% BSA, 2% 539 normal

(17) Immunofluorescence Staining of Paraffin Sections Step by StepFrontiers in NeuroanatomyNovember 9, 2020Sami Zaqout, Lena-Luise Becker, Angela M. Kaindlthe region around one, several, or all sections) is marked using a hydrophobic barrier pen (PAP pen; ab2601, Abcam, Cambridge, United Kingdom) or a nail polish (Figure 2B). The sections are then placed in glass

(18) Generation of Vascularized Neural Organoids by Co-culturing with Mesodermal Progenitor CellsSTAR protocolsJune 19, 2020Wörsdörfer P, Rockel A, Alt Y, Kern A, Ergün S.plate,Greiner,657160 "96-well plate, F-bottom",Greiner,655180 Cryomold,Tissue-Tek®,4565 PAP-Pen,Abcam,ab2601 Tissue Cassette,Süsse,100408 Paraffin (Histosec pastilles),Merck,1.11609.2504 2D Rocker 2D series

(19) Mcidas mutant mice reveal a two-step process for the specification and differentiation of multiciliated cells in mammalsDevelopment (Cambridge, England)March 15, 2019Lu H, Anujan P, Zhou F, Zhang Y, Chong YL, Bingle CD, Roy S.staining, slides were thawed and dried before drawing borders around the sections with a PAP pen (Abcam, ab2601). The slides were then fixed with 4% PFA for 15 min at room temperature in Coplin jars (all subsequent

(20) Lamins Organize the Global Three-Dimensional Genome from the Nuclear PeripheryMolecular CellSeptember 1, 2018Xiaobin Zheng, Jiabiao Hu, Sibiao Yue, Lidya Kristiani, Miri Kim, Michael Sauria, James Taylor, Youngjo Kim, Yixian Zhengformamide, the slides were dried and the area containing the mESCs was marked using a PAP-pen (Abcam, ab2601). The mESCs were blocked with 4% BSA in 1× SSCT for 30 min at RT, followed by incubation at RT for 2

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